Nine genetics had been predicted regarding the SD partNew SD area for O. luzonensis maps ranging from eyeless and you can 171M23F towards LG 12 (Tanaka mais aussi al. 2007). We performed further linkage research and you will acquired a few men recombinants to own this region. You to male got a beneficial recombination breakpoint anywhere between OluX2-8 and you can OluX2-twenty five, and the other got a good breakpoint ranging from OluX3-34 and you may OluX4-6, refining this new SD area so you can ranging from OluX2-8 and you may OluX4-six ( Figure 1A ).
(A) Genetic and you can real chart of intercourse-determining nations into the X and you can Y chromosomes. 1, normal XY men; dos, XX female; 3 and you may cuatro, recombinant men. Blue column, places produced from the newest Y chromosome; yellow, throughout the X-chromosome. Red-bar, BAC clones; blue pub, fosmid clones. Black arrows suggest predict genes. (B) RT–PCR affairs out of PGs (PG1–9) from the XX and you will XY human body trunk in the 0 months Nettsted her immediately following hatching. Meters, dimensions marker.
We built a beneficial BAC collection of an enthusiastic XX seafood and you may a fosmid collection of good YY seafood, therefore generated real charts of your SD area of the X and you may Y chromosomes. This region was wrapped in several BAC clones (OluBXKN2 and you may OluBXKN1) on the X chromosome sufficient reason for 7 fosmid clones (OluFY13-step 1, OluFY24-step one, OluFYstep 18-1, OluFY3-step 1, OluFY8-step one, OluFY7-1, and you can OluFY29-1) into Y chromosome ( Profile 1A ). Continua a leggere